Dr. Carola Cavallo of the Rizzoli Orthopedic Institute Publishes a Study on Platelet-Rich Plasma in the Journal of Bone and Joint Surgery
Dr. Cavallo and colleagues from Italy’s Rizzoli Orthopedic Institute recently published a study in the Journal of Bone and Joint Surgery titled “Comparison of Platelet-Rich Plasma Formulations for Cartilage Healing.” The study examined the effect of different platelet-rich plasma (PRP) formulations on in vitro human chondrocytes. Three formulations were prepared: 1. PRP with low concentrations of platelets and leukocytes (P-PRP); 2. PRP with high concentrations of platelets and leukocytes (L-PRP); and 3. Platelet-poor plasma (PPP). Then, chondrocytes from osteoarthritic cartilage were exposed to the formulations, and their cell proliferation, matrix production, and gene expression were evaluated.
The study demonstrated that all three formulations stimulated chondrocyte proliferation, though P-PRP induced greater cell growth at one week compared to the other two formulations. As for evaluation of gene expression, the study found that P-PRP stimulated greater expression of markers of chondrocyte anabolism. L-PRP, on the other hand, stimulated cytokines of catabolic pathways as well as gene expression leading to greater production of hyaluronan than P-PRP. The study suggests potential for the application of PRP for cartilage repair, however further evaluation of the optimal cellular composition and blood component ratios of platelet-rich plasma are needed to determine the most efficacious formula.
For more of the latest research on regenerative medicine and platelet-rich plasma be sure to attend the 6th Annual TOBI: The Orthobiologic Institute PRP & Regenerative Medicine Symposium on June 12-14, 2015 in Las Vegas. www.prpseminar.com Save $300 OFF Registration with Promo Code: TOBIBLOG