The Orthobiologic Institute (TOBI) Alumni Arnold Caplan Publishes New Study in Plastic and Reconstructive Surgery:

Mar 17

The Orthobiologic Institute (TOBI) Alumni Arnold Caplan Publishes New Study in Plastic and Reconstructive Surgery:

Arnold Caplan, The Orthobiologic Institute (TOBI) Alumni, and colleagues recently published a new article in Plastic and Reconstructive Surgery titled Human Epineural Sheath Conduit Augmented with Human Mesenchymal Stem Cells as a New Biologic Construct Supporting Peripheral Nerve Regeneration: A Preliminary Report. The study assessed the feasibility of a new biologic construct for nerve regeneration – human epineural conduit (hEC) consisting of human epineural sheath (hES) filled with 3-4 million human mesenchymal stem cells (hMSC) – on peripheral nerve injury repair in nude rats.


Sciatic nerve defects measuring 20mm were created in 24 nude male rats. The rats were divided into four experimental groups: Group 1 – no repair; Group 2 – autograft; Group 3 – hES filled with saline; and Group 4 – hEC. Outcomes were assessed with sensory pinprick and motor toe-spread tests at 1, 3, 6, and 12 weeks. Somatosensory evoked potentials, gastrocnemius muscle index, histomorphometry, and immunostaining were performed at 12 weeks.


Clinical evaluation and somatosensory evoked potential analysis confirmed sciatic nerve recovery in groups 3 and 4 with outcomes comparable to nerve autograft repair. Immunostaining showed presence of the hMSC in the conduit at 12 weeks post-implantation. Quantitative nerve and muscle histological analysis were still in progress at the time of this preliminary report.


The results of this study suggest the feasibility of hEC in the treatment of peripheral nerve injury. The functional outcomes following the use of hEC were comparable to the golden standard of autograft repair. Human epineural conduit is a promising new technology for regeneration of long gap nerve defects which utilizes the neurotropic properties of hES and immunomodulating properties of hMSC. Although the study provides evidence for potential benefit, further animal trials are needed to validate such claims and determine safety and efficacy for human trials.


For more of the latest research on regenerative medicine and hands-on musculoskeletal ultrasound cadaver lab with Dr. Jay Smith, be sure to attend the 6th Annual TOBI: The Orthobiologic Institute PRP & Regenerative Medicine Symposium with Cadaver Lab June 12-14, 2015 in Las Vegas, Save $300 OFF Registration with Promo Code: TOBIBLOG


Article link:

Leave a Reply

Your email address will not be published. Required fields are marked *